Journal: Biosensors

Article Title: Rapid Microfluidic Biosensor for Point-of-Care Determination of Rheumatoid Arthritis via Anti-Cyclic Citrullinated Peptide Antibody Detection

doi: 10.3390/bios14110545

Figure Lengend Snippet: Variation in OD intensity with anti-CCP Ab concentration for 2nd Ab-HRP reagents with different dilution factors. Error bars represent the range, calculated as the difference between the maximum and average values across three independent measurements.

Article Snippet: The anti-cyclic citrullinated peptide antibody (100 ng/mL = 30 IU/mL), sample diluent, and antibody diluent, as well as the 2nd antibody conjugate HRP (2nd Ab-HRP), were sourced from FineTest Inc. Additionally, 1% bovine serum albumin (BSA), diluted from a 10% stock solution was used as a blocking agent to reduce nonspecific binding, with phosphate-buffered saline (137 mM sodium chloride, 2.7 mM potassium chloride, 8.1 mM sodium hydrogen phosphate, 1.5 mM potassium dihydrogen phosphate), a wash buffer, and PBS containing 0.5% Tween 20 (PBST) used for rinsing steps.

Techniques: Concentration Assay

Journal: Cell reports

Article Title: Neurokinin-1 Receptor Signaling Is Required for Efficient Ca 2+ Flux in T-Cell-Receptor-Activated T Cells

doi: 10.1016/j.celrep.2020.02.054

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse / rat / human Substance P (rabbit polyclonal), Cy3 , Bioss USA , Cat # bs-0064R-Cy3.

Techniques: Functional Assay, Recombinant, Staining, Avidin-Biotin Assay, Blocking Assay, Plasmid Preparation, Inhibition, Fluorescence, SYBR Green Assay, Activation Assay, Bicinchoninic Acid Protein Assay, In Situ, Enzyme-linked Immunosorbent Assay, Activity Assay, Labeling, Software

Journal: Science Advances

Article Title: Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions

doi: 10.1126/sciadv.abb6595

Figure Lengend Snippet: ( A ) Schematic of HTP transfection with a photo perforation transfection system (PTS) was used. ( B ) Representative images showing HTP transfection. Nuclei were stained by DAPI (blue), and the HA-tagged HTP was stained by FITC-avidin (green). Scale bar, 100 um. ( C ) Quantification of transfection efficiency compared with Lip2000. *** P < 0.001 compared with Lip2000. Data are means ± SEM ( n = 3). ( D ) Schematic of DC modification with glycopolymers. ( E ) Representative images showing green fluorescence on the DC cell surface. Nuclei were stained by DAPI (blue) and biotin-labeled poly-(MAG) (pMB) by FITC-avidin (green). ( F ) Representative images showing the modified DCs incubated in complete medium for specified times (1, 3, and 7 days). ( G ) Viability of engineered DC over the 7-day period. Data are means ± SEM ( n = 3). N.D., not determined.

Article Snippet: Primary antibody hemagglutinin (HA) and FITC-conjugated secondary antibody were from Wuhan Boster Biological Technology Ltd. (Wuhan, China).

Techniques: Transfection, Staining, Avidin-Biotin Assay, Modification, Fluorescence, Labeling, Incubation

Journal:

Article Title: Herpes Simplex Virus Type 2 Induces Rapid Cell Death and Functional Impairment of Murine Dendritic Cells In Vitro

doi: 10.1128/JVI.77.20.11139-11149.2003

Figure Lengend Snippet: Subpopulation of HSV-2-infected BMDC exhibit DNA breaks shortly after infection. BMDC were infected with wt HSV-2 at an MOI of 4 or left untreated, incubated for up to 12 h p.i., and then double stained for HSV ICP8 (anti-ICP8 MAb 383 plus FITC-conjugated goat anti-mouse MAb) and for apoptosis (biotin-labeled dCTP followed by avidin-Texas Red). Shown are immunofluorescence microscope images of the same optical section of a population of HSV-2-infected DC collected 2 h p.i. to demonstrate staining for ICP8 (green) (A), nick end-labeled DNA breaks (TUNEL) (red) (B), and a double-labeled, HSV-2-infected, apoptotic BMDC (yellow). Arrow in panels A, B, and C indicates positive staining of an apoptotic, HSV-2-infected BMDC. Included were double-stained uninfected BMDC as a negative control (D) and DNase-treated uninfected DC stained with biotin-dCTP-avidin-Texas red as a positive control (E) for the TUNEL assay. Magnification, ×40.

Article Snippet: Cytospin slides were prepared and fixed as described above, blocked with 20% goat serum (Jackson ImmunoResearch), and then stained with antibodies against HSV ICP4 (M612446; BioClone), ICP8 (383; a kind gift from David Knipe, Harvard Medical School, Boston, Mass.), or HSV-2 glycoprotein D (gD) (910; Biodesign International, Saco, Maine), followed by FITC-conjugated goat anti-mouse MAb (Jackson ImmunoResearch).

Techniques: Infection, Incubation, Staining, Labeling, Avidin-Biotin Assay, Immunofluorescence, Microscopy, TUNEL Assay, Negative Control, Positive Control